The major challenges in TE are the preparation of porous scaffolds that can mimic special structures for cell attachment and growth. TE, which involves polymer scaffolds, tissue cells, and stimulation factors, has been widely used as an attractive therapeutic treatment for tissue defects. 1,2 In addition, the limitation of organ and tissue transplantations has driven the development of TE 3–6, in which new tissues are created from cultured cells and biomaterials. Introduction Tissue engineering (TE) is a multidisciplinary effort to reconstruct functional tissues and organs following trauma, end stage organ failure, and congenital abnormalities. These results indicate that selective enzymatic degradation has a potential application in scaffold manufacturing. Compared with the PLA scaffolds without any modification, the PLA scaffolds modified by poly- L-lysine exhibited a better biocompatibility to MEF cells. The results showed that the PLA scaffolds supported the attachment and growth of MEF cells. Cell viability was determined through scanning electron microscopy (SEM) and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2- H-tetrazolium bromide (MTT) tests. Meanwhile, the PLA scaffolds showed the largest swelling value of 157.4% after immersion in SBF solution for 14 days. The results demonstrated that the PLA scaffolds were degraded completely into harmless products in simulated body fluid (SBF) solution at a slow degradation rate, and the weight loss of the scaffolds could reach 80% after 8 months of in vitro degradation. PLA40 with 60% P(3HB- co-4HB) blending was used in in vitro degradation and mouse embryo fibroblast (MEF) cell culture tests. When the P(3HB- co-4HB) composition of PLA/P(3HB- co-4HB) blends was changed from 50 wt% to 60 wt%, the mean pore diameter of the porous PLA scaffolds increased from 64.15 µm to 80.01 µm, the porosity significantly improved from 68.45% to 79.88%, and the compressive modulus decreased from 9.34 MPa to 6.94 MPa. The porous morphology and properties of the scaffolds were investigated to clarify whether the microstructure of the biomimetic extracellular matrix is suitable for cell proliferation and differentiation.

In this study, poly(lactic acid) (PLA) scaffolds were prepared by selective enzymatic degradation using poly(3-hydrobutyrate- co-4-hydrobutyrate) depolymerase.

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